Unit content
Test crosses for inferring an unknown dominant-phenotype genotype
Under complete dominance, an individual showing the dominant phenotype can have genotype $AA$ or $Aa$. A test cross distinguishes these possibilities by crossing the unknown individual with a homozygous recessive partner, $aa$.
Why use $aa$? Every gamete from the tester carries $a$, so each offspring directly reveals which allele came from the unknown parent.
Case 1: the unknown parent is $AA$
The cross is
$$AA\times aa.$$
The first parent produces only $A$ gametes and the tester produces only $a$ gametes, so every offspring is
$$Aa.$$
All offspring therefore show the dominant phenotype.
Case 2: the unknown parent is $Aa$
The cross is
$$Aa\times aa.$$
The heterozygous parent produces $A$ and $a$ gametes in equal proportions, while the tester still produces only $a$.
Therefore
$$P(Aa)=\frac12,\qquad P(aa)=\frac12.$$
The expected phenotype ratio is
$$1\text{ dominant}:1\text{ recessive}.$$
Observing any recessive offspring proves that the dominant-phenotype parent supplied an $a$ allele and therefore cannot have been $AA$.
Finite samples create uncertainty
If a heterozygous parent happens by chance to produce several dominant-phenotype offspring in a row, the cross can temporarily look like the $AA$ case.
For example, if the unknown parent is actually $Aa$, the probability that four offspring all show the dominant phenotype is
$$\left(\frac12\right)^4=\frac1{16}.$$
So "all observed offspring are dominant" is evidence for $AA$, but it is not absolute proof unless the underlying biological system or sample size makes alternative explanations negligible.
A test cross is therefore an inference procedure: use a genetically informative tester so that offspring phenotypes reveal otherwise hidden allele transmission from the unknown parent.